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| Ansgar J. Pommer, SkinSysTec |
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Ansgar J. Pommer (PhD) was born on June 9th 1966 in Tauberbischofsheim, Germany. From 1987 to 1993, he completed his Chemistry degree at the Albert-Ludwig-University, Freiburg, Germany. During his final project, he worked with Prof. G. E. Schulz on the subject of Protein Crystallography. He moved to the University of East Anglia, Norwich, U.K. on a Wellcome Trust funded position and recieved his PhD working on a naturally occuring antibiotic in 1997. He then followed three more years of post-doctorate study in Protein Chemistry and Enzymatics. During this time, he wrote various Protein Chemistry articles for publication in peer-reviewed journals.In October 2000, he joined MelTec GmbH & Co. KG. There he established collaboration with the Magdeburg Clinic of Dermatology and was responsible for the skin project. This ultimately lead, in 2005, to the foundation of a new company SkinSysTec GmbH.
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Analyzing in-situ-proteomics by Multi Epitope Ligand Kartography (MELK) in skin research and beyond
Ansgar J. Pommer, SkinSysTec
Ansgar J. Pommer (1), Bernd Bonnekoh (2), Lars Philipsen (3), Raik Böckelmann (2), Harald Gollnick (2)
(1) SkinSysTec GmbH, (2) Clinic for Dermatology, Otto-von-Guericke-University, (3) MelTec GmbH & Co. KG, Magdeburg - Germany
We present our skin research-related platform of MELK robot technology. This break-through methodology allows to decipher the colocation of at least up to one hundred defined molecules (e.g. proteins) in subcellular spaces of biological specimens such as skin tissue sections or blood cell preparations. This opens new avenues for research and development in the fields of diagnostics, drug development and cosmetics.
MELK robot technology represents a highly advanced fluorescence microscopy. In principle it relies upon a sequel of i) incubation of a specimen with a fluorophore-labeled tag/antibody, ii) fluorescence imaging and iii) soft bleaching, allowing subsequent repeats of this cycling process. The point-precise overlay of the fluorescence images enables a mapping of the subcellular colocation of chosen molecular markers. Thus, a magnitude of molecular markers can be detected in a single specimen with a preserved histoarchitecture. This means a major advantage as compared to 1) conventional immunohistology (dealing rarely with more than 2 markers) or 2) cell- and tissue-disintegrating techniques alike conventional proteomics or array analyses.
Established applications of MELK robot technology are: a) the identification of pathophysiologic targets in inflammatory skin diseases, b) the monitoring of clinical treatments, c) the unprecedented in-situ-identification of rare cell types such as keratinocyte stem cells or d) the tracing of biological drugs (such candidates) for their tissue binding characteristics under the conditions of an ex vivo MELK biochip assay.
In front of this background the hardware and software components of our skin research platform of MELK robot technology and related service products will be presented. The workshop will be held in a questions & answers atmosphere and is addressing all interested representatives from research and industry in the fields of biotechnology, pharmaceutics, cosmetics and medicine.
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